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Subject: Меня конкретно нужно значение здесь staple .Rothemund used genomic ssDNA from the M13 phage as the scaffold strand (7249 nucleotides) and designed a set of short “STAPLE” strands to selectively bind to distant regions of the scaffold and fold it into a predesigned shape. gen. In 2006, the emergence of DNA origami32 transformed the landscape of structural DNA nanotechnology. The DNA origami method uses a number of short single-stranded DNA (ssDNA) oligonucleotides to direct the folding path of a long ssDNA “scaffold” strand. Rothemund used genomic ssDNA from the M13 phage as the scaffold strand (7249 nucleotides) and designed a set of short “staple” strands to selectively bind to distant regions of the scaffold and fold it into a predesigned shape.32 This assembly method results in near-quantitative yield for most 2D designs (Figure (Figure1F),1F), even with unpurified staples. Several groups successfully extended DNA origami fabrication to 3D33−36 and to the assembly of twisted37 and curved38 3D objects (Figure (Figure1F).1F). Other research groups have focused their attention on scaling-up DNA origami using the following methods: edge-to-edge base-stacking interactions between individual origami units,39 sequence-specific sticky end cohesion between individual units,40 “super origami”,41 and use of longer scaffolds for origami construction
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