Сам абзац к которому этот пункт относится не прояснил значения слова Focused.....
Мой вариант:
Целенаправленное пептидное картирование с жидкостной хроматографией-масс-спектрометрией для определения деамидирования
Если кто сталкивался, может есть устоявшийся термин??
Заранее благодарен

Principle
Belimumab is rapidly denatured, reduced and digested with trypsin, and then acidified with formic acid. The tryptic peptides are separated by RP-HPLC and detected by MS. The deamidation percentage of the peptide of interest is calculated from the peak area of extracted ion chromatogram (EIC) of deamidated peptide and that of its corresponding non-deamidated peptide.

Sampling
A reference standard is injected at the beginning and end of each sample set. Samples are injected once each for comparison to the reference standard.
Equipment and Equipment Parameters
The following equipment is used for the quantitation of deamidation in belimumab:
• Agilent 1200 HPLC system with Diode Array Detector (DAD).
• Agilent 6220 Accurate Mass Time of Flight (TOF) mass spectrometer.
• Agilent Zorbax 300 SB C18 (2.0 x 250 mm, 5 μm).
• Agilent Mass Hunter Qualitative Analysis Software.
Tryptic peptides are separated by reversed-phase HPLC using an Agilent Zorbax 300 SB C18 column with an Agilent 1200 HPLC system. The column is heated to a temperature of 40°C and a flow rate of 0.3 mL/min is used. A gradient is used for peptide elution with the following parameters: 16% B for 5 minutes, 16 to 36% B from 5 to 65 minutes. The column is washed with 80% B for 10 minutes and then equilibrated at starting conditions for 20 minutes
before the next injection. Mobile phase A consists of 10 mM ammonium formate in Milli-Q water and mobile phase B consists of HPLC grade acetonitrile. The HPLC elution is monitored by UV detection at 214 nm. Four percent formic acid is infused continuously with a flow rate of 0.1 mL/min through a post-column splitter, leading to a final concentration of 1% formic acid prior to the mass spectrometer. The HPLC elution is directly delivered into
the TOF mass spectrometer with electrospray ionization for mass detection. The mass spectrometer is calibrated with an Agilent tuning mixture prior to each analysis. Both centroid and profile data are acquired in a 2 GHz mode with a mass range of 300-3000 (m/z). Ion source conditions are as follows: 9 mL/min drying gas flow rate, 45 psi for nebulizer, 350°C gas temperature, 2500 V capillary voltage and 175 V fragmentor voltage. Mass spectrometry
data are processed using Agilent Mass Hunter Qualitative Analysis Software.

Еще раз огромное Вам спасибо, ой нет СПАСИБО!!
С уважением, ochernen