Subject: equilibrate pharma. To each tube add 50 µl of solution R1 and 50 µl of the appropriate dilution of thesubstance to be examined or the reference preparation. After each addition, mix but do not allow bubbles to form. Treating the tubes in the order S1, S2, S3, S4, T1, T2, T3, T4, T1, T2, T3, T4, S1, S2, S3, S4, allow to equilibrate at 37° C (water-bath or heating block) for 1 minute. Спасибо |
Предполагается, что содержимое пробирок, через 1 мин. после их погружения в термостат, достигнет равновесной т-ры 37 град. С |
Спасибоо за пояснение))) |
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