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Subject: Membranes were hybridized biol. Membranes were blocked for 1 hour in 5% nonfat dry milk in TBS-Tween and then hybridized using the following primary antibodies in 5% bovine serum albumin (BSA) TBS-Tween: phospho-Akt (Ser473), phospho-Akt (Thr308), Akt, phospho-S6 (Ser(240/4), phospho-S6 (Ser235/6), S6, phospho-PRAS40 (Thr246), PRAS40, phospho-Erk (Thr202/Tyr204), Erk, phospho-EGFR (Tyr1068), EGFR, phospho-HER3 (Tyr1289), HER3 (1:500-1:1000, Cell Signaling).Как в данно случае перевести hybridized? Спасибо!
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