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Subject: plasmid DNA formulated in water biol. An efficient IgE leader sequence (SED ID NO:65 nucleotide encoding SEQ ID NO:66 protein) was incorporated into the Env antigen gene sequences in order to improve expression.
The resulting modified and enhanced HIV-1Env-Fab DNA immunogens were codon-and RNA-optimized, followed by cloning into the pVax1 expression vector by GenScript (Piscataway, NJ), with subsequent large-scale production of these constructs.
The VH and VL genes (SEQ ID NOs:3 and 4, respectively) were inserted between the BamH1 and Xho1 restriction sites. Purified plasmid DNA was then formulated in water for subsequent administration into mice.
Как перевести "plasmid DNA was then formulated in water"?
Заранее спасибо!
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