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Subject: Rack Thawing Station biol. Seventy-five μL of 40% sample diluent were plated out in a 40% sample plate (Final 40% sample contains: 20 μM Z-block, 1 mM benzamidine, 1 mM EGTA, 40 mM HEPES, 5 mM MgCl2, 5 mM KCl, 1% Tween-20). One hundred ninety-five μL 1xSB17,Tw were plated out in a 1% sample plate. Ninety μL 1xSB17,Tw were plated out in a 1 to 10 dilution plate. One hundred thirty-three μL 1xSB17,Tw were plated out in a 0.005% sample plate. Samples were thawed for 10 min on the Rack Thawing Station in a 25°C incubator, then vortexed and spun at 1000x g for 1 minute.Спасибо! |
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http://www.biomicrolab.com/product/rack-thawing - вот этот продукт получается, "устройство для размораживания пробирок в штативе" |
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