Подскажите, пож-та, есть ли устоявшийся термин для перевода

pull-through PCR

Заранее спасибо

Pull-through PCR is a method to introduce restriction sites on the end of a PCR product. Many people will introduce their restriction sites on their original PCR primers, but this can sometimes cause non-specific binding or reduced PCR efficiency. The pull through method performs a PCR like normal and you purify the PCR product from a gel. From this PCR product, you THEN run a few additional PCR steps with primers that have your restriction sites on the ends.